Effect of Endurance Training and Calorie Restriction on Blood IGF-1 and IGFBP-3 in Rats.

• Main Authors: Chien-wei Chen, 陳建瑋
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/66163164234667575303

碩士 === 國立臺南大學 === 體育學系碩士班 === 99 === Purpose: The aim of this study was to investigate the effects of endurance running and calorie restriction on blood IGF-1, IGFBP-3 in male rat. Methods: Forty-eight male Wistar rats (7 weeks old) were randomly assigned into four groups, which were low intensity training group (LIT, n=12), high intensity training group (HIT, n=12), control group (CON, n=12), and diet control group (DCON, n=12). Animals in the HIT and LIT groups underwent endurance training at speed of 12m/min~22m/min and 15m/min~28m/min, respectively, on the treadmill 5 days per week for 8weeks. To match the body weight gain of the exercise group, the DCON rats received 80%~90% mean quantity of chow consumed by exercise groups. Other groups were fed with rodent diet and distilled water ad libitum. The body weight of each animal was measured twice a week. The end of 8-weeks training program, all animals (15 weeks old) were sacrificed. Blood and muscle samples were collected and stored for further serum marker and muscle enzymes assay, including insulin-like growth factor (IGF-1), insulin-like growth factor binding proteins-3 (IGFBP-3), citrate synthase (CS), 3-hydroxyacyl-CoA-dehydrogenase (3-HAD), phosphofructokinase (PFK) and lactate dehydrogenase (LDH) analysis. Result: Body weight gain was significantly higher in the CON group as compared with other three groups (p<.05). And soleus (SOL) weight was significantly higher in the CON group as compared with other three groups (p<.05). Extensor digitorum longus (EDL) showed no significant level among groups (p>.05). The CS and 3-HAD activity in SOL (predominantly type I fibers), it’s LIT and HIT groups showed a significantly higher than CON and DCON groups (p<.05). The PFK activity assay, DCON group was showed a nonsignificantly between four groups (p<.05). And the LDH activity, it’s LIT groups showed a significantly higher than CON and HIT groups (p<.05). In the DCON group, serum IGF-I concentration were significantly lower than other three groups (p<.05). Furthermore, the IGFBP-3 and IGF-1/IGFBP-3 showed a nonsignificantly between four groups. Conclusion: Regardless of the intensity of exercise, the study showed that the eight weeks endurance training can enhance the aerobic-oxidative metabolic enzymes activity of mitochondrial, but not significantly reduced IGF-1 concentrations in blood. In addition, calorie restriction can significantly reduced IGF-1 concentrations in blood. Furthermore, this study showed that the eight weeks endurance running and calorie restriction can not changed of IGFBP-3 level.