Study of TCP family in Orchidaceae

• Main Authors: Jui-ling Hsu, 許瑞鈴
• Other Authors: Wen-Chieh Tsai
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/68428729460336993069

碩士 === 國立臺南大學 === 生物科技學系碩士班 === 99 === The orchids involved varied types and the majority of orchids belong to the bilateral symmetry. Researches showed that the species with bilateral symmetry involved from radial symmetry. Bilateral symmetry plays important roles in plant pollination, because the bilateral symmetry could attract pollinator more efficiency than radial symmetry. Specifically, the impressive floral diversity of orchids has been attributed to adaption to specific pollinator. TCP genes involved in determination the symmetry of the flower and especially the symmetry of the petals in whorl 2. The TCP family contains two subfamilies and PCF subfamilies and TB1/CYC subfamilies separately. Within the TB1/CYC subfamily, the CINCINNATA (CIN) gene involved in the control of leaf growth and most likely CYC orthologs. The purpose of this study is isolation and characterization of TCP-like genes involving in flower zygomorphy in Orchidaceae. Previous researches showed that CYCLOIDEA and DICHOTOMA were expressed in dorsal petals to control the size, shape and stamen numbers in Antirrhinum. In this study, three TCP-like genes were isolated from Phalaenopsis equestris and named as PeCYC, PeCIN and PePCF. Southern blot analysis showed that TCP-like genes is single copy gene in P. equestris. RT–PCR was adopted to investigate the spatial and temporal expression patterns of these genes. Results showed that PeCYC is expressed in young flower bud stage. The PeCIN was expressed in all floral organs. The PePCF expression patterns were detected in all developmental stages buds and all vegetative tissue.