| Summary: | 碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 99 === Grouper in Taiwan is of high value, but nervous necrosis virus infection causes
100% mortality. Our laboratory had developed a good expression system to produce
virus-like particles that induced immune functions. In this study, cells producing
monoclonal antibody against the virus-like particles were used to induce BALB/c mice production of high titer ascites. The ascite from the H1 cells generated 6000-fold of antibodies higher than cell culture, using enzyme-linked immune-sorbent assay, although relatively less than the previous monoclonal cells of mX, 8000-fold. In the electron microscopy, the ascite antibody bound to various mutants of virus-like particles. Using the polymerase chain reaction to amplify fragments of IgG cDNAs, we will clone and express such cDNAs to efficiently produce the desired monoclonal antibodies.
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