| Summary: | 碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 99 === In order to discover bioactive secondary metabolites, we have studied the chemical constituents from the organic extracts of soft coral Sinularia flexibilis. This study had led to the isolation of eighteen natural compounds 1–18, including seven new cembrane-type diterpenoids, flexibilisolides C–G (1, 2, 3, 6, 7), flexiblilisin C (5), 11,12-secoflexibillin (4) along with eleven know compounds. The structure of compounds 1–18 were established by detailed spectral data analysis (IR, MS, 1D, 2D NMR) and by comparison of the spectral data with those of the related known compounds.
The cytotoxicity of compounds 1–6, 8–14, 16–18 against the Hela (human cervical epitheloid carcinoma), SK-Hep1 (human liver carcinoma), and B16 (human melanin carcinoma) tumor cell lines were screened. Compounds 1, 9, 12, and 13 showed moderate activity toward the Hela and SK-Hep1 tumor cells. Compound 12 also showed moderate activity toward the B16 cells, and compound 14 showed activity toward the Hela cells. On the other hand, compounds 1, 9, 13, and 17 were found to show significant activity against the accumulation of the pro-inflammatory iNOS and COX-2 protein at 10 μM. Compounds 2–4, 7, 8, 10, 14, 16 were found to show activity against the accumulation of the pro-inflammatory iNOS protein at 10 μM.
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