The contamination status of nonylphenol and octylphenol in groundwater in pig herds and their impacts on pig immune cell function

• Main Authors: Tai-Shun Chiou, 邱泰舜
• Other Authors: Ming-Tang Chiou
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/16991211608664631085

碩士 === 國立屏東科技大學 === 獸醫學系所 === 99 === Nonylphenol (NP) and octylphenol (OP), two well-known environmental hormones (EHs), have been detected in Taiwan. Current studies revealed the Kao-Ping River is one of the polluted areas. The goals of this project were to determine the pollution level of NP and OP in groundwater and pigs collected from pig herds beside the Kao-Ping River, and evaluate the effect of these pollutants on immune functions of porcine alveolar macrophages (AM) and peripheral blood mononuclear cells (PBMC). The experiment was divided into three parts. Firstly, to determine the NP and OP concentration in groundwater that was collected from pig herds. Second, to detect the NP and OP concentrations in blood samples obtained from pigs raised on these herds. Third, to evaluate the impact of NP and OP on the AM and PBMC immune functions in vitro. The results shown, NP and OP were detected from groundwater collected from varies pig herds. These two pollutants also detected from the blood samples of pig. Cell viability was significant decreased when AM and PBMC exposed under 10 ppm OP after 6 hours (p＜0.05). Down regulation of AM phagocytosis function was detected under treated with NP or OP with 10, 1, and 0.1 ppm after 24 and 48 hours (p＜0.05). PBMC proliferation ability was suppressed after exposed into 10 ppm NP or OP for 6 hours (p＜0.05). In addition, NP and OP could induce AM and PBMC apoptosis rather than necrosis. On the other hand, AM secretion tumor necrosis factor-α (TNF-α) ability was significantly enhance after exposed NP 10 ppm 6 hours (p＜0.05), whatever, AM stimulation with OP or NP 1 and 0.1 ppm after 6 hours, secretion TNF-α ability was significantly suppressed (p＜0.05). In contrast, neither NP nor OP co-cultured with PBMC, the interferon-γ secretion was unaffected. These results indicate that NP and OP could detect from groundwater and accumulated in the pig blood and impaired AM and PBMC functions.