Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus

• Main Authors: Yang Hsilan, 楊喜蘭
• Other Authors: Liao Minghui
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/21900644641376599918

碩士 === 國立屏東科技大學 === 獸醫學系所 === 99 === Staphylococcus aureus is the most common pathogen that causes nosocomial or community-acquired infections. S. aureus causes severe necrotizing infections of the skin, soft tissues, and lungs. Antimicrobial resistance is an increasing public health problem worldwide. Controlling methicillin-resistant Stapyhlococcus aureus (MRSA) remains a primary focus of most hospital infection control programs. The purpose of this study was to understand and analyse the relationship between molecular epidemiology and antibiotic susceptibility of MRSA. From January to December 2009, methillin resistance was investigated among a total of 439 S. aureus isolated from teaching hospital in Pingtung. MRSA isolates accounted for 47.8%(n=210) of all isolates, methicillin- susceptible Stapyhlococcus aureus (MSSA) isolates accounted for the other 52.2%(n=229). Specimen source included pus, respiratory tract, blood, urine, and others. Susceptibility testing was performed by a broth microdilution method. Pulsed-field gel electrophoresis (PFGE) was used for molecular typing of MRSA isolates, DNA was digested with the enzyme SmaI. The MRSA resistance rates to penicillin, ampicillin/sulbactam, clindamycin, and erythromycin were higher than 80%, that all were susceptible to vancomycin, linezolid, and teicoplanin. Ten antimicrobial resistance patterns were found, of which five accounted for 95.7%(n=201) of the MRSA isolates. The PFGE revealed 148 different patterns, were grouped into 20 types, designated A through T. Of all the MRSA isolates, 76.2%(n=160) were assigned to type A, E, G, J, N, O, P or T. These results could be helpful for understanding molecular epidemiology and antimicrobial resistance patterns characteristics of MRSA isolates from patients. The results obtained from this study can provide important information on the prevention and control of MRSA infections.