Development of Waterwall Cooling-Type Continuous-Flow PCR Chip

• Main Authors: Yu-Wei Ko, 柯又維
• Other Authors: Jyh-Jian Chen
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/22376132742656679194

碩士 === 國立屏東科技大學 === 生物機電工程系所 === 99 === Traditionally, the biochemical samples are cultured in Petri dishes. After completing the experiment, excessively used sample must be disposed. In order to save the cost and miniaturize the system, this research is to develop a micro-domain heating chip with waterwall cooling channel. The commercial software CFD-ACE+TM is utilized to simulate the thermal fields of the chip. There are two different constant temperature regions designed within the chip. Between these two isothermal areas, there is a waterwall cooling channel and the fluid in the channel can be used to control the temperature of the chip. The effects of various fluid flow velocities on the temperature distribution are examined. Simulation result shows that when the fluid flow rate in the channel becomes slow, it will be more easily heated by the isothermal areas with high temperatures. This makes the fluid temperature near the outlet getting higher and then the surface temperature of the chip is non-uniform. The optimal flow rate is found and it can be apply to the experiment. In our experiment, the chip system mainly consists of three parts. They include a PDMS-glass chip, two heating module and the fluid channel used to control the chip temperature. A PMMA channel for fluid flowing is fabricated under the chip. The heating module comprises two heaters that can be thermal controlled. When two different isothermal regions are created at the chip surface, we make use of the fluid which flows in the waterwall channel between the two heaters in order to cool the chip. Then three different isothermal zones can be created on the chip. The result shows that the fluid is easily heated by isothermal area, the effects of various flow rate in the channel on the surface temperature is noticeable. The obvious heat convective effect inside the cooling channel can be observed with the increasing of the fluid velocities. The large areas of the various isothermal regions are shown at the chip surface. Experiment results are compared with the simulated data, and it shows a similar trend. In the future we may establish several isothermal areas in the chip, and it could be applied in the fields of cell culture, drug screening or polymerase chain reaction in the chips.