| Summary: | 碩士 === 國立高雄海洋科技大學 === 水產養殖研究所 === 99 === This study was aimed to find out the effects of dietary supplemented carotenoids and garlic powder on growth, survival rate, antioxidant capacity and non-specific immune of barramundi, Late calcarifer. Eleven experimental diets supplemented with two types of carotenoid and garlic powder : astaxanthin, β-carotene, and garlic powder, at four concentrations : 0, 80, 160, and 320 mg/kg were feed to the fish for twelve weeks. No differences in growth and survival rate of the fish among treatments were found after 12-week rearing. Serum total antioxidant status (TAS). Serum antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidases (GPx), glutathione reductase (GR)] and serum transaminases [alanine aminotrans- ferase (ALT). asparatate aminotransferase (AST)] were chosen as indices of fish antioxidant capacity. There were no differences in TAS and GR between treatments and the control fish. The CD-fed fish had lower AST, ALT, and SOD activities than the control fish. The GPx of fish fed 160 and 320 mg/kg astaxanthin diets were significantly higher than those of fish fed β-carotene , garlic powder and the control diet. There were significantly different in GPx between astaxanthin treatment and the control diet.
Non-specific immune response (phagocytic activity, respiratory burst , lysozyme activity), superoxide dismutase and glutathione peroxidase activity were evaluated. Carotenoid and non-oxidized oil treated fish showed significantly higher respiratory burst activity compared with the oxidized oil groups. The phagocytic activity of head kidney leukocytes of fish fed 80 mg/kg β-carotene (BI) containing diets were significantly higher than those of fish fed astaxanthin, garlic powder and the control diet. However, no significant differences in phagocytic activity were observed between fish fed the astaxanthin, β-carotene and garlic powder supplemented diets. The serum lysozyme activities of fish fed garlic powder diets were significantly higher than those of fish fed the oxidized oil groups. Significant differences in lysozmye activities were found between fish fed garlic powder and the oxidized oil groups. Decreasing trends in SOD activities with increasing dietary astaxanthin and β-carotene were also observed. There were significant differences in SOD activity between fish fed the astaxanthin, β-carotene and the oxidized oil group. However, no significant differences in SOD activity were observed between fish fed garlic powder supplemented diets and the oxidized oil group. GPx activities of fish fed astaxanthin and β-carotene were significantly higher than those of fish fed the oxidized oil group. There were not significantly different in GPx activities between fish fed garlic powder supplemented diets and. the oxidized oil group.
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