| Summary: | 碩士 === 國立宜蘭大學 === 生物機電工程學系碩士班 === 99 === The purpose of this thesis was to integrate the viewpoints of behavioral toxicity and bioavailability into the observation of valve behavioral response of clams Corbicula fluminea exposed to copper （Cu） to develop a clam-based real-time bio-monitoring system for assessing Cu concentrations in aquatic environments. The developed process of this system was mainly divided into two phases. Firstly, the principles of water chemistry and Cu bioavailability were integrated into a toxicodynamic-based Hill model to derive a Cu - BLM - Corbicual model. The derived Cu-BLM-Corbicula model and the fitted valve daily opening / closing rhythm function from bioassay experiments were linked to compile a virtual instrumentation-based programmatic mechanism for simulating the valve behavioral response rhythm exposed to Cu. The simulated windows of the valve rhythm in response to copper were represented in a personal computer （PC） by employing a graphic control programmable language （LabVIEW software）. In the second phase, the observed data of the valve behavioral rhythm of clams exposed to Cu and the fitted valve daily rhythm function built in the database were employed to indirectly obtain the time-varying proportion of clams in response to Cu. From the computation of the inverse function of Cu-BLM-Corbicual model, the waterborne Cu2+-activity {Cu2+} can be indirectly estimated to further evaluate the site-specific waterborne Cu ion concentration [Cu2+] under various water quality conditions. The above computational mechanism was compiled by a LabVIEW software that displayed all profiles of the time-varying concentration of [Cu2+] in real-time visual windows. The performance results of the developed C. fluminea-based bio-monitoring system reveal that this system has a better assessing range while waterborne Cu concentration is below 100μgL-1. In this study, the compensatory mechanism of temperature-specific and pH-specific were incorporated into the developed C. fluminea-based bio-monitoring system to precisely and completely reflect the suitability for practical environmental statuses. The developed C. fluminea-based bio - monitoring system can offer a better and real-time measuring mechanism for quantifying Cu toxic effects on animals that was useful to manage the water quality in clam farms.
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