1D, 2D and 3D Thin Cell Layer Culture in Protocorm-like Bodies, Flower Stalk Bud and Apical Bud of Plantlet of Phalaenopsis

• Main Authors: Yu-Wen Chen, 陳郁文
• Other Authors: Chien-Yuan Kao
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/73698187811496300591

碩士 === 國立宜蘭大學 === 園藝學系碩士班 === 99 === Thin cell layer（TCL）system originated about 40 years ago with controlled development of flowers, roots, shoots and somatic embryos on tobacco pedicel longitudinal TCLs. Since then TCL technology have been successfully used in micropropagation of many ornamental plant species which in vitro regeneration was not successful using conventional methods. Thin cell layer system consists of explants of a small size excised from different plant locations, either longitudinally (lTCL) or transversally (tTCL). Phalaenopsis is one of the most important economic crops in Taiwan. Although many techniques have been applied in Phalaenopsis, such as micropropagation, virus detection and virus-free plantlets production. TCL technology remain as a untouched field in Phalaenopsis related area of research and industrial application. This study is the first report based on thin cell layer excised from protocorm-like bodies (PLBs), apical buds and flower stalk buds of Phalaenopsis. Various tTCLs thickness sectioned by microtome in their different plane of 1 dimension (1D), 2 dimension (2D) and 3 dimension (3D) were successfully generated. These various tTCLs were cultured in NDM medium supplemented with different concentrations of BA. In 1D tTCLs, 200 μm tTCLs excised from all materials used show that the highest ratio of PLBs formation. In 2D tTCLs, 200×200 μm and 500×150 μm tTCLs sectioned from PLBs and flower stalk buds respectively show the highest ratio of PLBs formation. In 3D tTCLs, 200×200×200 μm tTCLs excised from PLBs were sliced successfully and their PLBs regeneration could be observed. In this study, TCL technique is successfully applied in PLB, flower stalk bud and apical bud of Phalaenopsis and advance from 1D TCL by hands as reported in literature to 3D TCL in the material of Phalaenopsis.