The study of the regulatory mechanism for the aquaporin 5 gene expression

碩士 === 國防醫學院 === 生物化學研究所 === 99 === To date, thirteen mammalian aquaporins (AQPs) have been identified.AQP5 is selectively expressed in Salivary gland, lacrimal gland, sweat gland and lung alveolar epithelial cells. Xerostomia, the result of the decrease in secretion of saliva by salivary gland, is...

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Main Authors: Yu-Sheng Lin, 林育聖
Other Authors: Shih-Ming Huang
Format: Others
Language:zh-TW
Published: 2011
Online Access:http://ndltd.ncl.edu.tw/handle/39517199138852067424
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spelling ndltd-TW-099NDMC01070012015-10-28T04:07:29Z http://ndltd.ncl.edu.tw/handle/39517199138852067424 The study of the regulatory mechanism for the aquaporin 5 gene expression 探討調控 aquaporin 5 基因表現之機轉 Yu-Sheng Lin 林育聖 碩士 國防醫學院 生物化學研究所 99 To date, thirteen mammalian aquaporins (AQPs) have been identified.AQP5 is selectively expressed in Salivary gland, lacrimal gland, sweat gland and lung alveolar epithelial cells. Xerostomia, the result of the decrease in secretion of saliva by salivary gland, is a complication of diabetes mellitus. It is not clear whether the reduced saliva production is due to decrease AQPs expression in salivary gland. However, the correlation between blood glucose concentration and AQP5 expression has not been reported. In this study, we used various cell lines as a model to investigate whether AQP5 gene expression is regulated by glucose concentration, and identify which transcription factors that may be involved in the regulation. Here, we demonstrated that AQP5 was induced by glucose which may cause the hypertonic stress. Either 5-Aza-CdR or TSA was able to modulate AQP5 gene expression in specific cell lines, such as H1299 and HaCaT cells, implies the functional role of epigenetic regulation. AQP5 gene was also up-regulated by the knockdown of DNMT3a or DNMT3b, suggesting that DNMT3a and DNMT3b are involved in the stable hyper-methylation of AQP5 gene. We further identified two transcription factors, Sp1 and C/EBPbeta enhanced the promoter activity of AQP5 gene in A253 cells. Taken together, our data suggest that the regulation of AQP5 gene expression is not only mediated through the epigenetic modification, primarily through the DNA hyper-methylation, but also mediated through the response to environmental change for the recruitment of specific transcription factors onto its promoter. Shih-Ming Huang Yu-Juei Hsu 黃世明 許育瑞 2011 學位論文 ; thesis 63 zh-TW
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description 碩士 === 國防醫學院 === 生物化學研究所 === 99 === To date, thirteen mammalian aquaporins (AQPs) have been identified.AQP5 is selectively expressed in Salivary gland, lacrimal gland, sweat gland and lung alveolar epithelial cells. Xerostomia, the result of the decrease in secretion of saliva by salivary gland, is a complication of diabetes mellitus. It is not clear whether the reduced saliva production is due to decrease AQPs expression in salivary gland. However, the correlation between blood glucose concentration and AQP5 expression has not been reported. In this study, we used various cell lines as a model to investigate whether AQP5 gene expression is regulated by glucose concentration, and identify which transcription factors that may be involved in the regulation. Here, we demonstrated that AQP5 was induced by glucose which may cause the hypertonic stress. Either 5-Aza-CdR or TSA was able to modulate AQP5 gene expression in specific cell lines, such as H1299 and HaCaT cells, implies the functional role of epigenetic regulation. AQP5 gene was also up-regulated by the knockdown of DNMT3a or DNMT3b, suggesting that DNMT3a and DNMT3b are involved in the stable hyper-methylation of AQP5 gene. We further identified two transcription factors, Sp1 and C/EBPbeta enhanced the promoter activity of AQP5 gene in A253 cells. Taken together, our data suggest that the regulation of AQP5 gene expression is not only mediated through the epigenetic modification, primarily through the DNA hyper-methylation, but also mediated through the response to environmental change for the recruitment of specific transcription factors onto its promoter.
author2 Shih-Ming Huang
author_facet Shih-Ming Huang
Yu-Sheng Lin
林育聖
author Yu-Sheng Lin
林育聖
spellingShingle Yu-Sheng Lin
林育聖
The study of the regulatory mechanism for the aquaporin 5 gene expression
author_sort Yu-Sheng Lin
title The study of the regulatory mechanism for the aquaporin 5 gene expression
title_short The study of the regulatory mechanism for the aquaporin 5 gene expression
title_full The study of the regulatory mechanism for the aquaporin 5 gene expression
title_fullStr The study of the regulatory mechanism for the aquaporin 5 gene expression
title_full_unstemmed The study of the regulatory mechanism for the aquaporin 5 gene expression
title_sort study of the regulatory mechanism for the aquaporin 5 gene expression
publishDate 2011
url http://ndltd.ncl.edu.tw/handle/39517199138852067424
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