Study on the Chemical Constituents of a Sponge (Terpios hoshinota)-associated Marine Cyanobacterium GI-1

• Main Authors: Shin-Tong Jhu, 朱心彤
• Other Authors: Chung-Kuang Lu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/81396740087559977312

碩士 === 國立東華大學 === 海洋生物科技研究所 === 99 === Sponges are well known to host diverse microbes and represent a rich source of bioactive secondary metabolites. Terpios hoshinota, a cyanobacteriosponge, caused the coral “black disease” at Green Island, off the southeastern coast of Taiwan. We successfully isolated a cyanobacterium, strain GI-1 from T. hoshinota samples. The GI-1 is axenically cultivated under controlled condition in our lab. One new cyclic tetrapeptide; cyclo-Ile-Pro-Leu-Pro (1), one known cyclic tetrapeptide; cyclo-Ile-Pro-Leu-Pro (2) and fourteen known diketopiperazines (3-16) were isolated from the mass culture of GI-1. Twelve of Fourteen diketopiperazines are proline-containing cyclic dipeptides, including cyclo-Pro-Tyr (3), cyclo-D-cis-4-Hyp-L-Phe (4), cyclo-4-Hypro-Phe (5), cyclo-D-4-Hypro-L-Leu (6), cyclo-4-Hypro-Leu (7), cyclo-4-Hypro-Pro (8), cyclo-Pro-Ala (9), cyclo-D-Pro-L-Ala (10), cyclo-Pro-Val (11), cyclo-D-Pro-D-Val (12), cyclo-Pro- Ile (13) and cyclo-Pro-Leu (14). The structures of 1-16 were elucidated by NMR and mass data. In biological activity assays, the activity of compound 16 showed weak anti-imfammatory NO activity.