| Summary: | 碩士 === 國立東華大學 === 生命科學系 === 99 === The side effect of chemotherapy, radiotherapy or hormone treatment on breast cancer therapy still causes the breast cancer patients suffering great pain. The sensitized and synergistic compounds are searching for relief this side effect and increase the sensitivity of breast cancer for therapy. N-methyl-actinodaphine (MA, Cinnamon purified compound) with anti-oxidation activity can inhibit the bacterial endotoxin and cancer growth, and Polyalthia longifolia var. pendula has anti-inflammatory, antibacterial and nerve stabling properties. However, the sensitizing effects of MA and 16-hydroxycleroda-3,13-dien-15,16-olide (HCD Polyalthia longifolia var. pendula purified compound) with tamoxifen (hormone therapy drug) need to be explored.
The aim of this study was to investigate the inhibitory effect of natural compound (MA and HCD) on breast cancer cells MCF-7(ER+) and MDA-MB-231(ER-) using MTT assay. The IC50 of MA and HCD was 15 and 25 uM, respectively. The flow cytometry data showed that the subG1 of cell cycle was increased after MA and HCD treatment using PI staining, implying that MA and HCD could induce MCF-7 and MDA-MB-231 apoptosis. The combinatory effects of with MA and HCD with tamoxifen were increased the subG1 of cell cycle in both cell lines. Furthermore, according to the results of RT-PCR found that p53 and BAX gene expressions were also significantly increased while BCL-2 was decreased with treated time. Additionally, BAX increased and BCL-2 decreased in protein levels were observed. MA and HCD caused both breast cancer cell lines MCF-7 and MDA-MB-231 to apoptosis by the mitochondrial pathway. Two natural purified compounds possess the potential for developing as a sensitizer to enhance the efficacy of chemotherapy.
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