| Summary: | 碩士 === 國立東華大學 === 生命科學系 === 99 === Abstract
The macromolecules of protein, DNA and RNA could not permeate into cells from outside plasma membrane. Scientists found the peptides which contain more basic amino acids, could deliver macromolecules into living cells. The peptides were called cell-penetrating peptides (CPPs). In 2006, we have demonstrated CPPs could deliver macromolecules into plant cells. In this study, we delivered plasmid DNA into different species of animal cells in a noncovalent manner via CPP. Based on the transport of cyanine 3 (Cy3)-labeled plasmid DNA, we confirmed that CPPs could deliver plasmid DNAs into animal cells in a noncovalent manner. Afterward, we transferred the report gene encoding green florescent protein (GFP) into animal cells and assessed the green fluorescent signal by a fluorescent mircroscopy. Furthermore, we explored the cytotoxicity of CPP by the MTT assay and we proved that CPP was not cytotoxic. Thus, CPP would become a new tool in gene delivery.
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