| Summary: | 碩士 === 國立嘉義大學 === 微生物免疫與生物藥學系研究所 === 99 === Notopterygii Rhizoma, the dried rhizome and root of Notopterygium incisum Ting ex H.T. Chang or Notopterygium forbesii Boiss (Umbelliferae), is a famous traditional Chinese medicine which has pain-relieving effects. It contains a variety of coumarins, polyacetylenes, and phenolic compounds. Thirteen compounds were isolated from the dried rhizome of N. forbesii, including stigmasterol, β-sitosterol, fucusterol mixture, pregenolone, isoimperatorin, notopterol, notoptol, nodakenin, bergapten, bergaptol, phenethyl ferulate, hydroxyphenethyl anisate, ferulic acid, and chlorogenic acid. Eighteen commercial herbal medicines of Notopterygii Rhizoma were analyzed by HPLC and GC/MS. The contents of notopterol and isoimperaorin were significantly different among the commercial herbal medicines. Ultrasonic extraction with 70% EtOH for 2 hours of the commercial herbal medicines of Notopterygii Rhizoma had resulted in good outcomes on extraction. The data of GC/MS analysis showed the quality of Notopterygii Rhizoma were corelated with the content of monoterpeoids.
The 50% DPPH radical scavenging concentration (SC50) of chlorogenic acid, ferulic acid, and bergaptol were 77.29±0.32, 93.48±1.61, and 95.64±2.39 μM, respectively. These compounds were firstly examined for their cytotoxicity on RAW 264.7 macrophages by MTT assay, and their properties of inhibiting percentage on NO releasing by LPS-induced RAW 264.7 macrophage were evaluated. The results showed phenethyl ferulate and notopterol have inhibition on LPS-induced NO production in RAW 264.7 cells, and all compounds didn’t have cytotoxity. In conclusion, we have succeddfully isolated thirteen compounds from root of Notopterygii Rhizoma. Notopterol and phenethyl ferulate exhibited anti-inflammatory property of Notopterygii Rhizoma. The results also suggested that the rhizome of N. forbesii had anti-inflammatory activity and anti-oxidative activity. The quantitative information of each compound in N. forbesii may provide a standard for quality control on the related products.
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