Overexpression of Arabidopsis methionine sulfoxide reductase B7(AtMsrB7) enhanced tolerance to paraquat in transgenic rice plants

• Main Authors: Mei-Ping Hsu, 徐玫瓶
• Other Authors: Rong-Show Shen
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/99088488769536268615

碩士 === 國立嘉義大學 === 農藝學系研究所 === 99 === The gene fragment containing AtMsrB7 driven by CaMV35S promoter was transferred into rice plants to determine the function of MsrB7. The transgenic plants were obtained by Agrobacterium transformation. After selection by hygromycin, the genomic DNA were isolated from these transformants. The foreign gene was integrated into rice genome confirmed by Southern blot analysis. The expression of foreign gene was also proven by RT-PCR. The phenotype of these transformants was no significant as compared with wild type. Moreover, there is no delay flowering phenomena appeaed in these transgenic plants. The leaves of transgenic and wild-type plants appeared withered and brown after paraquat treatment. However, the chlorophyll contents of transformants were more than those of wild type. These results implied that AtMsrB7 enhanced little tolerance to oxidative stress in transgenic plants. The results of 3,3-diamino-benzidine (DAB) staining also indicated that the hydrogen peroxide level of transformants accumulated less than that of wild type. The peroxidase activity in the MsrB7ox plants was also higher than that in the wild type. These results indicated that the oxidative-removed ability of transgenic plants were higher than that of wild type. There is no significant difference of glutathione S-transferase avtivity between transgenic and wild-type plants. These results suggested that the glutathione S-transferase might not involve in the detoxification mechanism in rice plants. Combined all of the results, the overexpression of AtMsrB7 enhanced little tolerance to paraquat in transgenic rice plants.