Optimizing the condition of an HPLC-MS-MS for determination of C-reactive protein synthesized from human aortic endothelial cells

• Main Author: 張嘉良
• Other Authors: 劉敏瑛
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/98908444526919605560

碩士 === 國立彰化師範大學 === 化學系 === 99 === Atherosclerosis is the early pathology of cardiovascular disease. According to clinical studies, oxidized low density lipoprotein plays an important role in cardiovascular disease. If the concentration of low density lipoprotein in human blood is too high, they will penetrate into endothelial cell and will be oxidized by free radicals released from endothelial cells, smooth muscle cells and macrophages to form oxidized low density lipoprotein. It causes the immune response and atherosclerosis. Many reports indicated that C-reactive protein participated in the process of inflammation. C-reactive protein can be found in patient’s blood, and it’s concentration positively correlates with the risk of the cardiovascular disease. C-reactive protein is primarily produced by liver, new findings have shown that C-reactive protein is also produced by other diseased tissues. In this study, in vitro oxidized low density lipoprotein was used to stimulate human aortic endothelial cells. It can directly detect C-reactive protein released from endothelial cells by using enzyme-linked immunosorbant assay (ELISA) and optimized LC-MS-MS conditions.