Comparison of gene expression measurement by microarray using different preprocessing methods and Real-Time PCR

• Main Authors: Guan-cheng Chen, 陳冠丞
• Other Authors: Li-ching Wu
• Format: Others
• Language: en_US
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/33967643463681712044

碩士 === 國立中央大學 === 系統生物與生物資訊研究所 === 99 === Observation and analysis of gene expression are very important issues. There are many different methods and technologies to measure gene expression. Recently, the microarray technology has been widely used to measure gene expression because of its high-throughput data and relative low cost. But gene expression measured by different preprocessing methods of microarray may be variant. The difference between preprocessing methods may confuse scientists. So, we assume the gene expression measured by real-time polymerase chain reaction methods as a standard expression to compare gene expression measurement by microarray using different preprocessing methods.