| Summary: | 碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 99 === In this research, we aimed to establish an effective anaerobic digestion process condition which have ability to treat swine wastewater and produce renewable energy. Increases in methane (CH4) production were quantified when swine waste anaerobic digest with different condition. The anaerobic digestion experiments were conducted on flask (400 ml) in batch mode and lab-scale anaerobic digester (20 L) in continuous mode. In flask experiments was to treating swine waste under different pH adjustment and temperature by organic loading rate 20 gCODl-1d-1. The pH 8 and 35 ℃ showed the greatest methane production (186 mLd-1), a 58% increase from the control. In lab-scale anaerobic digester experiments, hydraulic retention times (HRT), temperatures and organic loading rates (OLR) were adjusted. Result showed that when inlet TCOD concentration about 12,000 mgl-1, it could methane production capacity achieved 4.6 Ld-1 (CH4 = 65.8%) at HRT 8 days. Temperatures and organic loading rates were also found feasible up to 36% of methane production. Polymerase chain reaction analysis of sludge in combination with denaturing gradient gel electrophoresis (DGGE) was used to determine changes in the composition of the bacterial and archaeal community during waste water treatment. Phylogenetic analysis showed that the sequences of DGGE were close to sequences of the Proteobacteria, Firmicutes, Methanothrix and Methanosphaerula. etc. The specific occurrence of these bacterial and archaeal species varied mainly with different condition. The investigation proved optimal operation parameters for anaerobic digestion, and it could application in full-scale reactors in agriculture field for clean energy.
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