| Summary: | 碩士 === 國立交通大學 === 分子科學研究所 === 99 === Recently, more and more researches focus on using nanoparticles for biomedical detection and as therapeutic tools, tracing their fate in organisms, or even used them for drug deliver. Generally speaking, a good biomarker should have the characteristics of low cytotoxicity, high emission intensity and specific peaks and easily surface functionalization, etc….
This thesis studied two different nanoparticles in bio applications. In the first part of the work, we applied malachite green to modify gold nanoparticle surface and transferred them into Hep G2 cell by electroporation. Then the Raman signal mapping were taken from the cells to determine the nanoparticle distribution inside the cells. We also used hGH to change the connection path with Hep G2 cells and compare the results with the malachite green. And the second part is that the entire experiment processes were repeated with the gold nanoparticles replaced with nanodiamonds.
The third part was to construct a microfluidic channel by using PDMS replica molding method. We investigated the correlation between the flow rate and the Raman signals when gold nanoparticles and nanodiamonds were pumped into the microfluidic channel respectively. It is possible to combine these two different nanoparticles with microfluidic channel to develop an automation biochip and to achieve real time detection in the near future.
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