| Summary: | 碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 99 === Macrophage play an important role in modulating the function of human body, and foam cell differentiating from macrophage is also the major factor in Atherosclerosis. In the present day, many artificial bio-implant have been designed and applied to many category. So it is an vital issue to consider the interaction between macrophage and artificial bio-implant surface. In our research, we use silicon based substrate to build nanodot arrays ranging from 10-nm to 200-nm, and seed macrophage isolating from mouse peritoneal on nanodot arrays. Then we further differentiae macrophage into foam cells by LDL.
In the morphology experiment, the outcome of cell adhesion area are that 10-nm, 50-nm,100-nm increase respectively 6.17%、25.7%、9.63% cell adhesion area compare with flat surface and 200-nm decrease visibly 14.74% ,accompanied with apoptosis-like. Foam cells increase respectively 7.11% and 12.4% cell adhesion area on 10-nm and 50-nm surface and decrease 6.5% and 7.8% on 100-nm and 200-nm surface compare with flat surface. We may imply that 50-nm surface has more bio-compatibility than 200-nm surface in terms of macrophage and foam cell . According to immunostaining, we found that 50-nm shows the more viculin and actin filament distribution in 50-nm surface than other nanodot size, which indicate that 50-nm surface promote cell adhesion and cytoskeleton organization. On the contrary, 200-nm surface hinders cells from adhesion and inhibits the organization of cytoskeleton. In terms of cell lamellipodia length, 100-nm and 200-nm shows the most extended lamellipodia of all nanodot size. It reveals that macrophage and foam cell execute their innate immune function on the surface of 100-nm and 200-nm. Then we perform a cell viability test, judging from the statistics, macrophage and foam cells seeding on 50-nm surface have the most viability and the less viability on 200-nm surface. The outcome accord with the previous inference that 50nm surface has the more bio-compatibility than 200-nm surface.
In the gene experiment, we utilize RP-PCR to observe the gene expression of macrophage and foam cells. The outcome reveals that 100-nm and 200-nm surface have apparently inflammation gene expression for macrophage and 10-nm surface also have inflammation for foam cell.
In our outcome, we may infer that nanostructure can modulate macrophage and foam cell in cell growth, cell density and cell spread area, immune function with size dependently. Macrophage and foam cell on 50-nm surface have less inflammation and more adhesion area. Possible application of nanostructure on the artificial implants is expected.
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