| Summary: | 碩士 === 國立交通大學 === 生物科技學系 === 99 === Novel aspect of nanotechnology and biotechnology has been extensively exploited to develop multimodal contrast agent for cancer detection. We have developed highly specific dual modality T2 weighted MR-optical imaging agents (MnMEIO-antiMUC4-Cy777-mPEG NPs) for early detection of pancreatic cancer. A novel PEG derivative, methoxypoly(ethylene glycol)-ethylamine-silane (mPEG-NH2-silane), was strategically designed and synthesized such that amine group of NPs remain buried deep inside the entangled mPEG chains. Furthermore, in order to achieve nearly neutral surface charge of MnMEIO NPs, nonconjugated passively masked NH2 was further blocked by cysteine. Dynamic light-scattering (DLS) measurements revealed that the mPEG-NH2-silane coated MnMEIO NPs has a relatively narrow size distribution with mean size of 39.3 ± 2.2 nm which was increased 55.9 ± 5.6 nm upon conjugating anti-MUC4 mAb, and Cy777. The relaxivity values (r1 and r2) of the MnMEIO-Ab-Cy777-mPEG NPs measured at 20 MHz and 37.0 ± 0.1 牵C are 35.7 ± 1.5 and 216.1 ± 4.4 mM-1s-1, respectively. In vitro MR image shows significant contrast enhancement in MUC4 positive cell line. Therefore, we believe the highly sensitive and specific MnMEIO-antiMUC4-Cy777 NPs introduced in the present thesis could potentially be used for early detection of pancreatic cancer.
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