Traditional Chinese medicine preparations containing the doping substances - ephedrines

• Main Authors: Szu-Yun Hu, 胡斯雲
• Other Authors: Mei-Chich Hsu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/06675518838583962401

碩士 === 國立體育大學 === 運動科學研究所 === 99 === Ephedrae Herba is one of the prescriptions in Traditional Chinese Medicine (TCM) preparations for releasing symptoms due to cold. The major ingredients of Ephedrae Herba, ephedrines, including ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, and methylephedrine are banned/monitoring substances by the World Anti-Doping Agency (WADA). In previous study, the amount of ephedrine per therapeutic single-dose of Sho-seiryu-to was 2.98 mg and the mean of maximum urinary concentration (Cmax) of ephedrine was 4.67 μg/mL following individuals took single dose of the preparation. Based on this result, it is speculated that an individual who takes a single-dose of TCM preparation that contains 6.38 mg of ephedrine may lead to anti-doping rule violation. The purpose of this study was to determine the amounts of ephedrines per therapeutic single-dose in TCM preparations for colds by HPLC. We collected the TCM preparations from three GMP pharmaceuticals companies in Taiwan, and determinted the content of ephedrines n these preparations. The results show that 34% of those TCM preparations had more than 6.38 mg ephedrine in a therapeutic single-dose, such as Ma-hsing-kan-shih-to (10.269 mg), Mahwang-to (9.018 mg) and Ma-huang-fu-tzu-hsi-hsin-to (6.578 mg) from A company, Dah-ching-long-to (10.008 mg), Ma-hsing-kan-shih-to (7.215 mg), Mahwang-to (7.614 mg), Ma-huang-fu-tzu-hsi-hsin-to (15.818 mg) and Kakkon-to (10.518 mg) from B company, Sho-seiryu-to (9.514 mg), Chinfeitsao-san (20.792 mg), Ma-hsing-kan-shih-to (8.663 mg), Mahwang-to (34.970 mg) and Ma-huang-fu-tzu-hsi-hsin-to (31.262 mg) from C company. Therefore, the excretion of ephedrine in urine might exceed 10 μg/mL after taking only a single dose of these TCM preparations. In conclusion, it is estimate that all the TCM preparations for cold that contain E over 6.38 mg may cause a positive ephedrine test result, which violates the WADA rules. We suggest that athletes should avoid take these TCM preparations for cold.