Development of Molecular Imprinting Polymers for Analysis of PAHs in Mainstream Cigarette Smoke

• Main Authors: Wei-LunHo, 何緯倫
• Other Authors: Ta-Chang Lin
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/23992167271322924757

博士 === 國立成功大學 === 環境工程學系碩博士班 === 99 === In this study benz[a]pyrene (BAP) was selected as the template molecule. Several different imprinted polymers were synthesized and studied for their characteristics and application efficiencies in environmental sampling. After preparation of the molecular imprinted polymer (MIP) and non-imprinted polymer (NIP), their capacities, selectivity, recovery, and breakthrough were determined by SEM analyses, differential scanning calorimeter (DSC), and the BET surface properties test. Results show that some MIPs have good selectivity toward benz[a]pyrene from among a mixture containing 16 PAHs. Analytical results show that the combination of using 4-vinylpyridine (4-VP) as a functional monomer, along with dimethacrylate (EDMA) as the crosslinker resulted in a higher adsorption capacity 12.80 ± 1.3 μg/g than other MIPs. The SEM micrographs reveal that the surface characteristics of particles were related to the different crosslinkers. The DSC test indicates that both the functional monomer and crosslinker were important controlling factors of imprinted polymers and NIPs. In addition, the surface properties of imprinted polymers and NIPs demonstrate that MIP pore size was larger that that of NIP, implying that the pore size may affect the capacity and selectivity of MIP. In this study, Kentucky reference cigarettes 3R4F were sampled. The benz[a]pyrene levels obtained by Soxhlet extraction was 11.5 ng/cig, respectively. All the experimental results suggest that MISPE can more effectively adsorb BaP among the 16 different PAHs in mixtures, reduce background interferences and increase signal resolution as compared to traditional extraction techniques. Additionally, using MISPE cartridges in sample pretreatment reduces both the analysis time and the amount of organic solvents used. In addition, using MISPE may also extract toxic target analytes from a complex sample selectively, and more effectively than traditional Soxhlet extraction. The combination of MIP-4 and SPE for environmental sample pretreatment offers a useful tool for selective isolation of the mutagenic PAHs. This study is also the first to apply an MISPE cartridge in the analysis of trace level BaP in cigarette smoke.