| Summary: | 碩士 === 國立成功大學 === 生命科學系碩博士班 === 99 === P53 and its downstream factors control many biological functions, including aging, cell cycle progression, and cellular death. It is reported that p53 mutates in more than 50% of tumor, and it is found that p53 establishes its anti-tumorgenesis effect by co-operating with various factors, including p21, PUMA, and DRAM, thus it is recognized as tumor suppressor gene. However, human papillomavirus (HPV) infection inactivates p53 through the function of E6 oncoprotein in various cancer cell type, as in human cervical carcinoma cell. Therefore, regeneration of p53 and its function is regarded to be an important strategy to suppress tumorgenesis. In this study, we demonstrated that hinokitiol, a natural compound derived from the heartwood of cupressaceous plants, might limit tumor proliferation. Hinokitiol can reinforce p53 activity in HeLa cervical adenocarcinoma and induce cell cycle arrest, cellular senescence, and autophagic protein expression. Furthermore, hinokitiol also suppresses EGF-induced cellular migration by inhibiting the migration-related kinase activity, indicating the role of hinokitiol in anti-malignancy. Our results suggest that hinokitiol may be a promising reagent to suppress tumor proliferation and migration.
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