Development of Subcellular Biosensor

• Main Authors: Jing-Tong Huang, 黃景同
• Other Authors: Cheng-Chung Chang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/38098237634869070106

碩士 === 國立中興大學 === 生醫工程研究所 === 99 === The first section of this thesis is development of fluorescent sensors. The 3-position of the phenothiazine (PTZ) core was conjugated with dipicolylamine (DPA) and maleimide, respectively. The former product was designed to be a fluoroionophore and the later product to be a thio-reaction sensor. In the mean time, the 7-position of PTZ core was substituted with donor or acceptor functional groups. Hence, the optimum detection condition and their characters of fluoroionophore and thio-sensor were evaluated with ICT (Intramolecular charge transfer) and PET (Photon electron transfer) effect behaviour. The second section of this thesis is development of quantitative fluorescent pH sensor. This binary conjugate was constructed with a pH-responded green fluorescence enhancement fluorophore and a red fluorescence fluorophore whose emission property was inert to condition and acted as a control color of fluorometers. Furthermore, this fluorometer was used to stain the cell and quantitatively measure the pH value of organs in cell.