Prognostic variables, and clonality and pheno-typing by polymerase chain reaction and immu-nohistochemical methods in canine lymphomas

• Main Authors: Yi-Ping Yang, 楊伊平
• Other Authors: Shih-Chieh Chang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/80362054180068739171

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 99 === Lymphoma is the most common hematopoietic malignancy in dogs, and immu-nophenotype is one of the most important prognostic factors studied in the past decade. Polymerase chain reaction (PCR) for antigen receptor rearrangement (PARR) has ex-panded the diagnosis and clonality assay in canine lymphomas. The aim of this study was to define the prognostic variables among dogs that received different chemothera-peutic protocols, and to compare the sensitivity of immunohistochemical (IHC) staining with PCR assay in the determination of tumor lineage. Eighty multicentric and 10 primary cutaneous lymphomas were included. A single-agent doxorubicin protocol presented with a similar efficacy regarding the progression-free and survival times, whereas a significantly higher complete response (CR) rate, with the combination pro-tocol in dogs with multicentric lymphoma. Dogs with B-cell phenotype were 9 times as likely as dogs with T-cell phenotype to have a CR to chemotherapy in multicentric lymphomas (P = 0.089). Clinical substage, P-glycoprotein expression prior to chemo-therapy, and response to therapy were significantly associated with the survival time. Tumor lineage was determined in 66 lymphomas by IHC and PCR methods. As a result, multicentric lymphomas comprised 78.6% (44/56) B-cell and 12.5% (7/56) T-cell lymphomas, and 5 dogs (5/56; 8.9%) remained undetermined. All the 10 cutaneous lymphomas were of T-cell phenotype. Dual phenotype and genotype were separately found in two multicentric lymphomas. Overall, a higher sensitivity of IHC immuno-phenotyping than PCR clonality assay (93.4% versus 87.0%) in the determination of lineage was demonstrated in all lymphomas in the present study.