| Summary: | 博士 === 國立中興大學 === 動物科學系所 === 99 === The study was conducted to investigate the effects of dietary β-1,3-1,6-glucan supplementation on the reproductive performance, immunity and the resistance to coccidiosis of New Zealand White (NZW) rabbits. The change of blood biochemical values of NZW and Rex does from late gestation to lactation period was measured first. The results showed that the serum total protein, cholesterol and phosphorous concentration significantly (P<0.05) decreased at 27th day of gestation for the two breeds. Serum calcium and triacylglyceride concentration of NZW does were significantly (P<0.05) lower at 24th day of lactation. The serum glucose and total protein concentration of NZW does were significant (P<0.05) lower than Rex does. Alanine aminotransferase, aspartate aminotransferase, creatine kinase and lactate dehydrogenase activity of NZW does were significantly (P<0.05) increased with the progression of gestation and lactation. The change of Rex does was not as higher as NZW does but showed the same pattern. The endocrine and blood biochemical values of multiparous and primiparous NZW breeding does were measured in the second trial. The result showed serum ACTH, cortisol and progesterone were highest at 28th day of gestation. Estrodiol and progesterone were significantly (P<0.05) lower at 4th day of lactation. Serum total protein, albumin, total cholesterol and triacylglyceride were significantly (P<0.05) lower at 28th day of gestation. The effects of dietary β-1,3-1,6-glucan supplementation on reproductive performance and immunity of NZW rabbits was assessed in trial 3. Dietary supplementation of β-1,3-1,6-glucan showed no effect on reproductive performance of NZW does. 0.128% β-1,3-1,6-glucan supplementation significantly (P<0.05) decrease the feed intake compared with the control group at the first inclusion period (14th day of gestation to 28th day of lactation) but without significant difference after adaptation at lactation period. Dietary supplementation of 0.064% β-1,3-1,6-glucan significantly inhibited (P<0.05) delayed-type immune reaction at late gestation period. It also found significantly (P<0.05) increased serum IgM and IgG concentrations at the late lactation period. Percentage of peripheral lymphocyte subset CD4＋ for weanling pups was significantly (P<0.05) higher (P<0.05) for 0.064% β-1,3-1,6-glucan supplementation group. Serum triacylglyceride and cholesterol concentration of all treatments were (P<0.05) significantly lower at 28th day of gestation. Concentration of nonesterified fatty acid was increased with the progression of gestation and lactation for all treatments. Trial 4 investigates the effect of dietary β-1,3-1,6-glucan supplementation on the resistance of coccidiosis for NZW rabbits. Dietary β-1,3-1,6-glucan supplementation showed no adverse effect on feed intake compared with the inclusion of coccidostatics (lasalocid sodium) at the beginning of inclusion. The feed intake and average daily gain were significantly decreased (P<0.05) for growing rabbits after challenge with 3.0×105 oocysts, except for the group inclusive with coccidostatics (robenidine hydrochloride). The feed intake for the β-1,3-1,6-glucan supplementation group was significantly higher (P<0.05) than the lasalocid sodium inclusive group after challenge. The dietary β-1,3-1,6-glucan supplementation has the potential to increase immunity of rabbit and replace the coccidostatics.
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