| Summary: | 碩士 === 國立中興大學 === 物理學系所 === 99 === In this study Real-time convection PCR instrument, the design of a large amount of light received the optical signal of the intercepted system to read eight of the capillary system of two fluorescent properties. First of all, using ZEMAX optical software to simulate the imaging system, the system received 0.19 of N.A, the flux can be increased to 10 times. Image quality, the system of Radial energy distribution is 74%. Modulation transfer function at 100 lp / mm is 0.8, the center with the edge of the flux difference are 10%, the actual measured difference is 7%. Actually, measuring the concentration of 0.45μM of Fluorescein signal, and the total fluorescence intensity is about 10 times of the background value, The system of the flux is higher 10 times than directly received light.
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