Application of rumen bacterial isolates for production of biofuels from lignocellulose

• Main Authors: Ang-Hsuan Lee, 李昂軒
• Other Authors: Chieh-Chen Huang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/59456914940713885458

碩士 === 國立中興大學 === 生命科學系所 === 99 === In our previous study, a stable rumen-mimic bacterial consortia system that could act as functional union for biohydrogen and biofuels production was constructed. The result revealed that cellulolytic Clostridial strains were predominated in the system. In this study, to enhance the efficiency for simultaneous saccharification and fermentation (SSF) by Clostridium xylanolyticum Ru15 and Clostridium puniceum Ru6 were co-cultured to develop a dual microbial SSF system. The fermentative metabolites analyzed to isolate that including acetate, butyrate, ethanol and butanol. Since C. acetobutylicum strain 824 could efficiently utilize butyrate for butanol production. However, the co-culture system were use C. acetobutylicum strain 824 with the cellulolytic Clostridial strains that could degradation lignocellulose of napiergrass 12%, and degradation hemicellulose 11%, but that was nonsignificant additive of butanol production by SSF. Finally, in order to enhance butanol production and saccharification, we were enhanc the ability of sacharification of bacteria by synthetic biology. Our bacteria of synthetic could significant additive ability to degradation and saccharification lignocellulose.