Chlorella sorokiniana T89 cDNA annotation and functional gene analysis

• Main Authors: Jin-Mien Chiu, 邱靖棉
• Other Authors: 陳玉婷
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/dn6smj

碩士 === 國立中興大學 === 基因體暨生物資訊學研究所 === 99 === The heat-talent Chlorella sorokiniana T89 was cloned from Chiayi paddy field. The genomic DNA and cDNA annotated data was unpublished so far. The lipid content of C. sorokiniana T89 was up to 30% and rich in unsaturated fatty acids which were suitable as biodiesel and functional foods. Culturs of C. sorokiniana T89 at different growth stages were used for RNA extracted and converted into cDNA for future sequencing analysis. Then get the contigs sequence data by Illumina sequencing system. Contigs were predicted their function via sequence alignment by using BLAST tools from NCBI and KEGG to find gene functions. Among the annotated genes with differential expression, the superoxide dismutase (SOD) which plays an importante role at antioxidant pathways was selected for further cloning, expression and biochemical characterization. In the future, we can use the annotation database to study the genes involved in physiological pathway of C. sorokiniana T89 andto developict as a good resource of biodiesel.