| Summary: | 碩士 === 國立中興大學 === 化學系所 === 99 === Monoclonal antibodies, such as cetuximab(C225), herceptin, etc., have been utilized for target cancer therapy in clinic. In this study, C225 conjugated polymeric micelles with photosensitizer loading were prepared for active targeting photodynamic therapy (PDT). First, mixed micelle composed of poly(ethylene glycol)-b- poly(lactide) and maleimide-poly(ethylene glycol)-b-poly(lactide) with photosensitizer loading were prepared by solvent evaporation method. Next, the C225 antibody was conjugated to the micelle surface via thiol group and maleimide group coupling. Our results show that the photosensitizer-loaded C225-conjugated micelle can target selectively to the epidermal growth factor receptor (EGFR) overexpressed cancer cells with efficient cell killing ability after irradiation whereas most EGFR-negative cells survived under the same treatment. Thus, this active-targetable photosensitizer delivery system may have great potential for cancer therapy.
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