Synthesis and Characterization of Fe3O4 Nanoparticles and their Applications in Stem Cell Labeling
碩士 === 國立中興大學 === 化學工程學系所 === 99 === Magnetic resonance imaging (MRI) is a technique that can provide detailed signals of the internal organs of human body or test animals. Superparamagnetic iron oxide nanoparticles (SPION) can serve as the contrast agent in MRI. In this syudy, Fe3O4 magnetic nanopa...
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| Format: | Others |
| Language: | zh-TW |
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2011
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| Online Access: | http://ndltd.ncl.edu.tw/handle/99419497167196386460 |
| Summary: | 碩士 === 國立中興大學 === 化學工程學系所 === 99 === Magnetic resonance imaging (MRI) is a technique that can provide detailed signals of the internal organs of human body or test animals. Superparamagnetic iron oxide nanoparticles (SPION) can serve as the contrast agent in MRI. In this syudy, Fe3O4 magnetic nanoparticles (NPs) were prepared and characterized by trasmission electron microscope (TEM), x-ray diffraction (XRD), and fourier transform infrared spectrum (FT-IR).The cytotoxicity and cellular uptake of Fe3O4 NPs by mouse neural stem cells (mNSC) and rat adiposed-derived adult mesenchymal stem cells (rADAS) were evaluated. Results showed that Fe3O4 NPs were successfully synthesized. Cytotoxicity to mNSC and rADAS were not observed at lower concentrations (< 100 ppm). NPs could be taken up by the mNSC and rADAS. We found that culturing mNSC and rADAS on chitosan membranes increased the uptake of Fe3O4 NPs. We expected to use Fe3O4 NPs in stem cells labeling and tracking in the future.
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