The Sequence Study of Genes Involved in the Production and Secretion of Bacteriocin Weissella cibaria 110

• Main Authors: Cheng-Yu Tsai, 蔡承佑
• Other Authors: Han-Tso Lin
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/94762132984713532894

碩士 === 銘傳大學 === 生物科技學系碩士班 === 99 === Weissellcin 110 is a newly discovered bacteriocin in Weissella cibaria 110. In the previously study, the partcial amino acid sequence of weissellcin 110 has been illustrated, however, the full-length sequence of this bacteriocin is still lacking. In current thesis, the attempt to discovery the DNA sequencing of both flanking regions neighboring the known sequence region, that included the 5’ and 3’ sequence unknown regions of wessellcin 110, and the genes neighbored. Two strategies that (1) traditional inversed PCR with restriction enzyme and T4 DNA ligase pretreated circular genomic fragmented template (2) newly designed PCR with random and ATCG sequence -based linker primer was introduced to deal with the problem. The current data demonstrated that both two strategies are work to amplify a constant PCR product. The following cloning and sequencing experiments are proceeding.