High-Resolution Melting Analysis for Rapid Detection of BRAF and PIK3CA Gene Mutations in Colorectal Cancer

• Main Authors: Li-Ling Hsieh, 謝麗鈴
• Other Authors: Ta-Chih Liu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/47701699472255969206

碩士 === 高雄醫學大學 === 醫學研究所 === 99 === Epidermal growth factor receptor (EGFR) monoclonal antibody therapy is established in patients with wild-type KRAS colorectal cancer ; however, up to 50% of these patients do not respond to this therapy. To identify the possible causes of this treatment failure, we searched for mutations of several oncogenes involving in the EGFR-dependent signaling pathways. In this study, high resolution melting analysis (HRMA) was used to screen hot-spot mutations in the BRAF and PIK3CA genes in 182 colorectal cancer specimens. Direct sequencing was used to confirm HRMA results. Activating mutations were detected in 28.6% of KRAS (codon 12, 13), 1.1% of BRAF (V600E), 4.9% of PIK3CA (exon 9, 20) and 4.9% (KRAS and PIK3CA) of the 182 colorectal cancer specimens. HRMA provides a fast, high sensitivity, and valid approach to efficiently detect these oncogene mutations. Failure of EGFR antibody therapy in patients with wild-type KRAS colorectal cancer may result from activating BRAF or PIK3CA mutations.