The research of serum TNF-alpha and blood lead levels among the different types of TNF-alpha promoter polymorphisms in the lead workers and healthy controls.

• Main Authors: Chao-Yi Lin, 林昭宜
• Other Authors: Chuang, Hung-Yi
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/16463668788317077226

碩士 === 高雄醫學大學 === 醫學研究所 === 99 === Introduction and aims : The lead-induced injury is multiple organs in human, which the factors of the injury was considered oxidative stress, inflammation and functional NO deficiency. Some studies have shown that TNF-alpha?ncan induce extra-reactive oxygen species which are related with oxidative stress and inflammation. The aim of this study is to investigate the effects of oxidative stress, inflammation induced by lead exposure among the different types of TNF-alpha?? promoter polymorphisms. Methods : The lead-exposed subjects came from a lead factory and the controls collected from the public but no lead exposed. The blood lead levels were evaluated by AAS(Atomic Absorption Spectrophotometer) assay. The serum TNF-alpha???nlevels were detected by ELISA. The TNF-alpha?npromoter polymorphisms were analyzed by Specific primer-probes to run Real-Time PCR. Results : In this study, the lead-exposed subjects had higher blood lead levels and serum TNF-alpha?nlevels than those of the controls. The polymorphisms of the lead-exposed subjects and the controls were not significantly different. Dividing into 4, the highest blood lead level group had significantly higher serum TNF-alpha?nlevel. But The polymorphisms of the all groups of blood lead levels were not significantly different. With the multiple regression model, the rise of serum TNF-alpha?nlevel was only associated with the increased blood lead levels and the decreased BMI level. Discussion : The study has shown that exposure to lead induced TNF-alpha?nbut the TNF-alpha?npromoter polymorphisms did not effect the lead-induced TNF-alpha?nexpression. However, the other polymorphisms could effect the mechanism, which need further investigation.