Validation of the integrins as tumor markers of oral squamous cell carcinoma

• Main Authors: JUN-HSU TSAI, 蔡閏旭
• Other Authors: Chung-Ho Chen
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/87376243095569049415

碩士 === 高雄醫學大學 === 牙醫學研究所 === 99 === Background: Early detection of oral squamous cell carcinoma (OSCC) is helpful for better prognosis and survival rate . The identification and validation of OSCC biomarkers are still main stream of modern researches. Materials and methods: 55 Paired-OSCC/safe margin normal tissue samples were collected. Based on our previous microarray data, 4 integins candidates were selected. Subsequently, the real-time RT-PCR is applied to validate these candidates for the suitability of OSCC detection. All results were statistically analyzed with clinicopathological factors. Results: Integrin alpha 3(ITGA3)，Integrin alpha 5(ITGA5)、Integrin beta 1(ITGB1)和Integrin beta 6(ITGB6) were selected from up-regulated genes. The area under the Receiver Operator Characteristic curve (ROC curve) for ITGA3, ITGA5, ITGB1 and ITGB6 were 0.715 (95% confidence interval [CI] = 0.626~0.823, p = 0.004), 0.698 (95% CI = 0.597~0.800, p = 0.004), 0.640 (95% CI = 0.536~0.734, p = 0.011), and 0.657 (95% CI = 0.554~0.760, p = 0.005) for OSCC patients versus normal controls respectively. These results suggested that the ITGA3, and ITGA5 were significantly detected the OSCC under the control choice of normal tissue samples. No significant difference was found between each stage of AJCC oral cancer classification, gender, and between different years of contacting history of carcinogenic factors (alcohol, betel quid and cigarette) in relative gene expression of OSCC and their paired tissues. If we use ITGA3, ITGA5, ITGB1, and ITGB6 for detecting OSCC may be less limited to these clinicopathological features of OSCC. However, there was significant difference between different tumor location in ITGA3. Conclusion: ITGA3, ITGA5 are potential biomarkers for detection of OSCC.