| Summary: | 碩士 === 義守大學 === 生物技術與化學工程研究所碩士班 === 99 === Anisomeles indica (L.) O. Ktze grows mainly in subtropical regions. In Taiwan,it frequently appears in forest edge or abandoned ground of the low-elevationmountains. People often used it as folk medicine to treat rheumatoid arthritis,gastroenteritis, and other inflammatory diseases. However, very few studies reported on the inhibitory effects of A. indica on the proliferation, metastasis, migration and invasion of oral cancer cells. The statistic data from Department of Health show that the incidence and mortality rate of compatriots’ oral cancer patients ranked at the six of the compatriots top ten cancer diseases in Taiwan at 2009. The tumors of oral cancer have been proven clinically to be fast-growth, and are easier to metastasize to lung, lymph nodes and other parts. This study investigates the inhibitory effects of A. indica extract (AIE) on the growth, migration and invasion of FaDu human pharyngeal carcinoma cells and their related mechanisms.
The results of MTT assay for AIE on FaDu cells showed good cytotoxicity on FaDu cells (IC50 = 60 μg/ml at 24-hr treatment) in a dose-dependent manner. By the Flow cytometric analysis, AIE arrested FaDu cells at G2/M phase. Western blotting analysis showed that AIE induced the apoptosis of FaDu cells by down-regulating the anti-apoptotic Bcl-2 and Bcl-xL protein level and up-regulating the apoptotic proteins level of Bax, Bad and the activation of downstream procaspase-3. The results of Wound healing assay, Cell migration assay and Cell invasion assay demonstrated that AIE had significant inhibitory effects on the cell migration and invasion of FaDu cells in a dose-dependent manner. Western blotting analysis showed that the levels of p-P38, p-JNK, p-NF-κB, MMP-2, MMP-9 proteins and the NF-κB in nucleus decreased when the AIE dose increased. Moreover, the results of ELISA kit assay and gelatin zymography on the expression of MMP-2 and MMP-9 showed that both of the MMP-2 and MMP-9 are significantly decline when the dose of AIE increased. In conclusion, the above results illustrated that AIE can inhibit the growth, migration and invasion of the FaDu cells.
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