The application of Chinese medical herbs to the culture of hybridoma and the production of monoclonal antibodies against Japanese encephalitis virus E protein

• Main Authors: Yu-Ting Huang, 黃鈺婷
• Other Authors: Ya-Ching Lin
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/84654677268308615190

碩士 === 輔英科技大學 === 生物科技系碩士班 === 99 === In this study, the extracts from seven Chinese medical herbs were used for the culture of hybridoma E3. Different concentrations of the extracts were added to the media and then the growth of hybridoma E3 and the monoclonal antibodies against Japanese encephalitis virus E protein were examined. Our results show that in general herbal extracts at 0.5μl/ml were not conducive to the growth of hybridoma. The extracts of Agastache rugosa and Salvia miltiorrhiza Bge at concentrations below 0.5μl/ml promoted both cell growth and antibody production. Similarly, the extracts from the roots and leaves of Scutellaria rivularis Benth at concentrations below 0.1μl/ml promoted both cell growth and antibody production. The extracts of Angelica dahurica, Trigonella forenum-graecum L and Trichosanthes kirilowii Maxi at concentrations higher than 0.3μl/ml promoted antibody production but not for cell growth. The results suggest that high concentrations of herbal extracts have direct effects on increasing antibody production without the demand of cell reproduction, while increasing effects on antibody production at lower concentrations may be accompanied by cell proliferation.