Analysis Of Functional Components In Beer Lee And Its Antiobesity Activities

• Main Authors: Guan-Yan Wu, 吳冠彥
• Other Authors: Tsai-Hua Kao
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/56213613922144571020

碩士 === 輔仁大學 === 食品科學系 === 100 === Beer is one of the main alcoholic beverages in the world, which is rich in nutrition and contains various functional compounds. Beer lee is a by-product generated from beer brewing and composed with spent grains, spent hops and surplus yeast, which have been used as cattle feed or fertilizer. However, studies of the amount of functional compounds in beer lee are rare. The objectives of this research are to analyze the content and variety of prenylflavonoid and hop bitter acid in beer lee by using high performance liquid chromatography, and to discuss the antiproliferation, apoptosis, and antiadipogenesis activity of 3T3-L1 cell line as affected by ethanol extract from beer lee. Results showed that 4 of prenylflavonoid and 9 of hop bitter acid can be separated simultaneously in 29 minutes by employing a Thermo HyPURITY C18 column (150 mm × 4.6 mm I.D., 5 μm) with the gradient solvent system of phosphoric acid in water (pH 1.6) and acetonitrile at flow rate 1.5 mL/min and detection wavelength at 314 nm. Moreover, the variety of these compounds can be identified as isoxanthohumol, xanthohumol, 8-prenylnaringenin, 6-prenylnaringenin, cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone in beer lee. In cell proliferation test, the preadipocyte and adipocyte viability decreased by increasing the concentration of beer lee extract and showed a 50% inhibition at 7.3 μg/mL and 10.4 μg/mL. Apoptosis analysis showed that beer lee extract increased the number of apoptotic cells in adipocyte, but not in preadipocyte by FITC-Annexin V/propidium iodine apoptosis flow cytometry. Cell cycle analysis revealed that treatment of preadipocyte with beer lee extract caused cell cycle arrest at the G2/M phase, and the cyclin B1 expression also shown to be up-regulated. During differentiation of 3T3-L1 preadipocytes to adipocytes, the beer lee extract efficiently reduced adipocyte number and the generation of intracellular triglyceride by oil red staining and triglyceride assay respectively.