Manufacturing Technique and the Properties of Chitosan / Polylactide Composite Membrane

• Main Authors: Po-Ching Lu, 呂柏青
• Other Authors: Jia-Horng Lin
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/13156023474308856859

碩士 === 逢甲大學 === 纖維與複合材料學系 === 99 === Chitosan features good anticbacterial, biocompatibility and biodegrable. Glucosamine is chitosan’s biodegrable product which is nontoxic, and thus is widly used in biomedical field. The aim of this research was to explore the physical properties and cell culture compatibility of the chitosan/ Polylactide composite membrane as well as to determine the influence of the addition of the PLA net over the chitosan/PLA composite membrane. PLA fibers underwent the nonwoven manufacturing to form the PLA net, which was thermal-bonded and then cut into the same size. Chitosan dissolved in the 2% acetic acid to form the 5 wt% of chitosan solution, after which the solution was poured into the mould to soak the trimmed PLA net. Not until the PLA net completely soaked the chitosan solution, was it dry-frozen into the chitosan/ PLA composite membrane by a freeze dryer. The tension test, degradation test, cell culture compatibility test and in vivo were employed to determine the optimum clinical conditions for the chitosan/ PLA composite membrane. In the results of this research, the low melting PLA nets have various direction of fiber at 0°, 45°, 90°, 135° showed the fiber distribution was homogeneity. The air permeability of the PLA net was decreased 9.5% after Alkali treatment. In tensile test, earlier gelation (EG) composite membrane and later gelation (LG) composite membrane than non gelation have lower tensile strength were 21 % and 8 % respectively. In degradation ratio test, within a month, composite membrane with various gelatig type have different degradation ratio. The degradation ratio of the non gelation (NG) composite membrane, EG composite membrane, LG composite membrane were 44 %, 43% and 39 % respectively. In two months, they were 43 %, 49 %, 47 % respectively. In swelling ratio test, the NG composite membrane has lower swelling ratio were 51 % and 145 % than EG composite membrane and LG composite membrane respectively. In the other hand, the water content only of the NG composite membrane was increased 3.5 % and 15 % than EG composite membrane and LG composite membrane respectively. From SEM morphology have found the composite membrane contain amount of pore on the surface. Therefore, the water contact angle of the composite membranes were 0° when composite membranes at different type of gelation. In cell compatibility, low melting PLA net and composite membranes with various gelation type were exist cell around the samples. In addition, the cell almost was attached on well as a fusiform form. And the phenomenon means that the composite membranes have well cell compatibility via the optimum design of experimental parameters.