| Summary: | 碩士 === 大葉大學 === 生物資源學系碩士班 === 99 === Cordyceps militaris was a valuable dicinal fungi that posseses pharmacolugcal activities similar to Cordyceps sinensis. Several studies have demosertrated that Cordyceps militaris has multiple biological actiivities, including anti-cancer, anti-inflammatory and immunological stimulating activities. In recent years, Cordyceps militaris has been popular and cheaper than Cordyceps sinensis on the market. However, the biological activities of anti-allergy from Cordyceps militaris still remained unclear. This thesis is to investigate the anti-allergic activities by using different extracts from Cordyceps militaris. The results showed that the extracts did not induce cytotoxic effects of the mast cell lines at low concentration. In the mast cell line RBL-2H3, degranulation induced by Compound 48/80 experiments was significanty inhibited against histamine release. The percentages of inhibition by methanol and ethanol extracts are 92.55±7.7%, 68.34±8.5% in 100ppm, respectively. Furthermore, in this study, we used the orthogonal test and ultrasound assisted extraction of cordycepin in Cordyceps militaris, the the maximum rate of target content is 7.04 mg/g, representing 86.98% of total extract. Compare to the traditional extraction mothods, the efficiency increased about 20%.
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