Study of Different Elicitor Factors of Antrodia Cinnamomea Cultured on Cereal Medium

• Main Authors: Kao, Nien-Hua, 高念華
• Other Authors: Ko, Wen-Ching
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/27532018328411590032

碩士 === 大葉大學 === 生物產業科技學系 === 99 === Antrodia cinnamomea is on unique medicinal fungal species in Taiwan and only parasitizes inside the decayed heart wood of Cinnamonum kanehirae. According to recent researches, A. cinnamomea extract had medicinal effects in anti-cancer, anti-inflammation, anti-hypertension, anti-oxidation and diabetes mellitus. Its mein active ingredients are polysaccharides and triterpenes. However, the using modem cultivation technique, basswood produced triterpenoids content at around 0.8% but fruiting bodies of the same weight giving about 3% of triterpene. In this study, we would like to induce the formation of triterpenoids and ultrasound-assisted extraction by using orthogonal experiment of optimization with the extraction conditions. The results showed that among the herbal activator of triterpenoids, the best one are Ganoderma lucidumt. The cultivation for 45 days produced triterpenoids up to 1.13% ± 0.01, followed by the second Betel pepper (1.09%), and the third Magnolia officinalis (0.96%). In the cultivation for 15 days, G. lucidum gave the highest bio-diversion of triterpenoid (2.41). The chemical activator was Squalene, it triterpenoid content and bio-diversion were 1.18±0.08% and 2.55. In other factors, the moisture content and ventilation had significant effects, but in acute low temperature acute no significant effect. In ultrasound-assisted extraction, four variables of the extractive time (30,35,40,45 min), ethanol concentration (55,65,75,85 %), extractive temperature (40,50,60,70℃) and ultrasonication frequency (28,40,56,80 kHz). After analysis of variance (ANOVO), the ethanol concentration and ultrasonication extraction time had significant differences (p＜0.05). Through experimental design and analysis, the optimal conditions of riterpene extraction ethanol concentration up to 93%: 75%, extractive temperature: 60℃ and ultrasonication frequency: 28 kHz. The ultrasonication extraction time (only 35min) was much shorter than their soxhlet extraction and immersion extraction. Ultrasonic extraction was proved to be effective to shorten the extraction time and improve extraction efficiency.