| Summary: | 碩士 === 中原大學 === 生物醫學工程研究所 === 99 === The aim of study was to determine the effect of Light Emitting Diode (LED) on Vascular Endothelial Growth Factor (VEGF) of hyperglycemic burned-wound in rat. The effect that wavelength 630nm LED irradiation on VEGF in wound healing was investigated. During photo-stimulation, the energy was applied with a dose of 4 J/cm2 (14 mW x 280 sec). The hyperglycemic rat was induced in normal Wistar rats by single intraperitoneal injection (150 mg/kg of body weight) of alloxan monohydrate. The blood glucose level was measured at two days, one weeks and two weeks later after injection, when blood glucose greater than 250 mg/dL we call it hyperglycemic rats. After rats were anesthetized with pentobarbital (50 mg/kg, i.p.), a metal rod pre-heated to 100℃ was used and applied to the dorsal side of the rats for 20 sec. Four burn areas created with the average size of rats burn site were 1x1 cm2. In our studies separate rats in two groups, that normal and hyperglycemic burned rars groups. And then divide irradiation and non-irradiation groups for each. The wound healing were evaluated by histological sections which were counted the numbers of neutrophils, macrophages, vessels and fibroblasts and then wound tissue VEGF secretion from wound tissue were measured.
The results show that the number of neutrophils decrease from 458.7±59.9 to 260.7±28.4 about 43%, macrophages increase from 128±8.2 to 145.3±8 about 12%, vessels increase from 25.7±2.1 to 35.3.3±8 about 28%, and fibroblasts increase from 92.3±9.6 to 180.3±6.1 about 49% in normal burned rats after LED irradiation compared to non-irradiation control. The amounts of VEGF increase from 803. 2±89.5 to 809.2±41.5 pg/ml about 1% in normal burned rats after LED irradiation compared to non-irradiation control.
In hyperglycemic burned rats, the number of neutrophils decrease from 394.7±12.5 to 205.7±19.5 about 48%, macrophages increase from 104±6.2 to 105±3.6 about 1%, vessels increase from 19.7±2.5 to 20.7±1.5 about 5%, and fibroblasts increase from 134.7±19.6 to 162.3±23.1 about 17% after LED irradiation compared to non-irradiation control. The amounts of VEGF increase from 377.12±25.4 to 571.9±31.2 pg/ml about35% in hyperglycemic burned rats after LED irradiation compared to non-irradiation control.
The results showed that no matter for normal or hyperglycemic burned rats, LED irradiation show the effects as follows compared to non-irradiation control. Both of normal and hyperglycemic groups did not show significantly increase on the numbers of macrophages firstly. LED has more significantly effect in hyperglycemic wound tissue (about 35% increased) than normal (about 1% increased) on VEGF secretion secondary. LED irradiation promotes the fibroblasts proliferation in both normal and hyperglycemic burn wound thirdly. In conclusion we show that the 630nm LED irradiation promotes wound healing process in both normal and hyperglycemic burned wounds. It’s possibly enhances the activity of macrophages to secrete more VEGF, and then causes vessels proliferation. Finally, it provides the oxygen and nutrient to wound tissue and promotes wound healing process.
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