Biological Activities of Indolicidin and Its’ Analogues onFibroblast, Keratinocyte and Human Epidermoid Carcinoma

• Main Authors: Zhao-Cheng Ling, 林昭呈
• Other Authors: Jui-Hsiang Hsieh
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/36088668022791623928

碩士 === 中原大學 === 生物醫學工程研究所 === 99 === Many literatures indicated that indolicidin(IL), one of cationic antimicrobial peptides(CAPs) isolated from the cytoplasmic granules of bovine neutrophils, has broad-spectrum antimicrobial activity. It can inhibit the growth of bacteria, fungi and even viruses. A growing number of studies have shown that some of cationic antimicrobial peptides may own cytotoxic activity against cancer cells. We, therefore, try to investigate the cytotoxic activity of IL and its analogues against human epithelial carcinoma A431and normal human skin cells, HS68 and CRL2309. We vary the concentration of IL and its analogues and investigate the relationship between peptide concentration and cell toxicity. The suitable concentrations may be found to have high activity against human epithelial carcinoma but low cytotoxicity toward normal fibroblasts and keratinocytes. Three concentrations were tested: 60μM, 30μM and 10μM. The result showed that one of the IL analogue, IL-K7F89, has the highest anticancer activity to A431 and the lowest cytotoxic activities toward HS68 and CRL2309 at 10μM. By using PI stain and TUNEL assay, we also found that indolicidin and its analogues kill cells by direct necrosis but not inducing apoptosis. In addition, previous study had indicated that IL-K7F89 has higher antimicrobial but lower hemolytic activity than IL does. Our study further indicated that the IL analogue, IL-K7F89, will also be a good candidate for cancer treatment.