| Summary: | 碩士 === 中原大學 === 生物醫學工程研究所 === 99 === Many literatures indicated that indolicidin(IL), one of cationic
antimicrobial peptides(CAPs) isolated from the cytoplasmic granules of
bovine neutrophils, has broad-spectrum antimicrobial activity. It can
inhibit the growth of bacteria, fungi and even viruses. A growing number
of studies have shown that some of cationic antimicrobial peptides may
own cytotoxic activity against cancer cells. We, therefore, try to
investigate the cytotoxic activity of IL and its analogues against human
epithelial carcinoma A431and normal human skin cells, HS68 and
CRL2309.
We vary the concentration of IL and its analogues and investigate
the relationship between peptide concentration and cell toxicity. The
suitable concentrations may be found to have high activity against human
epithelial carcinoma but low cytotoxicity toward normal fibroblasts and
keratinocytes. Three concentrations were tested: 60μM, 30μM and 10μM.
The result showed that one of the IL analogue, IL-K7F89, has the highest
anticancer activity to A431 and the lowest cytotoxic activities toward
HS68 and CRL2309 at 10μM. By using PI stain and TUNEL assay, we
also found that indolicidin and its analogues kill cells by direct necrosis
but not inducing apoptosis. In addition, previous study had indicated that
IL-K7F89 has higher antimicrobial but lower hemolytic activity than IL
does. Our study further indicated that the IL analogue, IL-K7F89, will
also be a good candidate for cancer treatment.
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