Preparation of intelligent pH-responsive zwitterionic soft membranes and its bioadhesive properties

• Main Authors: Chun-Wei Huang, 黃俊瑋
• Other Authors: Yung Chang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/04746919204568439916

碩士 === 中原大學 === 化學工程研究所 === 99 === Abstract This work describes a novel tunable-bioadhesive soft membrane of pH-responsive 2-(N,N-dimethyl amino)ethyl methacrylate (DMAEMA) containing zwitterionic sulfobetaine methacrylate (SBMA). This novel soft membrane highly regulates general bioadhesive foulants through the adsorption of plasma proteins, the adhesion of human platelets and cells, and the attachment of bacterial. The copolymeric soft membranes exhibited controllable pH-dependent swelling behaviors and showed stimuli-responsive phase characteristics in the presence of cationic ions (H+ )or anionic ions (OH-). The interactions of these soft membranes with biomolecules and microorganisms were demonstrated by protein adsorption, cell adhesion, and bacterial attachment, which allowed us to evaluate their bioadhesive properties. An enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies was used to measure different plasma protein adsorptions on the prepared soft membrane surfaces. The attachment of platelets and the spreading of cells were only observed on polyDMAEMA-rich soft membrane surfaces. Interestingly, the incorporation of zwitterionic SBMA units into the polyDMAEMA soft membranes was found to accelerate the hydration of the bacterial attached surfaces and resulted in more rapid bacterial detachment. Such copolymer gel surface was shown to be potentially useful for triggered bacterial detachment. This work shows that the bioadhesive properties of poly(DMAEMA-co-SBMA) hydrogels can be effectively controlled via regulated ionic and zwitterionic molar mass ratios. The tunable-bioadhesive behavior of pH-sensitive poly(DMAEMA-co-SBMA) makes this biocompatible hydrogels appropriate for biomedical applications.