The Inhibition Effect for Chinese Herbs on TCDD Induction of CYP1A1in Human Hepatoma Cells

• Main Authors: Chung-Hao Chen, 陳中豪
• Other Authors: Ya-Fen Wang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/3up76b

碩士 === 中原大學 === 土木工程研究所 === 99 === Dioxin is notorious for its liver toxicology and procarcinogenesis because of its lipophilicity and highly accumulation through food web. Most in-vitro studies are focused on the inhibition effect for food extracts and medicine on TCDD induction of CYP1A1 in rat hepatoma cell (H4IIE) or human breast cancer cell (MCF-7). Little information can be referred to the inhibition effect for Chinese herbs on TCDD induction of CYP1A1 in human hepatoam cells. The DRE-Luciferase assay was applied in this study to evaluate the inhibition effect of Schisandra chinensis and Cinnamomum cassia extracts on TCDD induction of CYP1A1 based on three treatment modes: pre-treatment, cotreatment and post-treatment. The results showed that the highest inhibition effect for the pre-treatment among four concentrations (1, 10, 30, 50 μg/ml) was 15% for Schisandra chinensis aqueous extracts and 4% for Schisandra chinensis 90% alcoholic extracts, respectively. The highest inhibirion effect was 48% for Cinnamomum cassia aqueous extracts and 35% for Cinnamomum cassia 90% alcoholic extracts, respectively. Furthermore, Schisandrin B and Cinnamaldehyde were processed to investigate the inhibition effect on TCDD induction of CYP1A1. The results showed that the inhibition effect was among 8 ~ 27% and -113 ~ 35% for Schisandrin B and Cinnamaldehyde, respectively, for the three treatment mode. The preliminary findings demonstrated that Schisandra chinensis and Cinnamomum cassia extracts show promising potential as an agent to block the AhR metabolic pathway for 2,3,7,8-TCDD.