The relationship between adult allergy and single nucleotide polymorphism of human chitinase 3-like 1 gene

• Main Authors: Hui-Fen Liu, 劉惠芬
• Other Authors: Yu-Ching Li
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/73737049249400737455

碩士 === 中臺科技大學 === 醫學檢驗生物技術系碩士班 === 99 === Chitinase 3-like 1 (CHI3L1) is one kind of mammalian chitinase family proteins, as well as the 18th glycohydrolase family. Increased expression of CHI3L1 has been correlated with the presence of many kinds of different diseases, including cancers, autoimmune diseases, and chronic inflammatory conditions. According to further genetic analysis, some European researchers found that CHI3L1 gene was the susceptible gene of allergy. There was an indication that single nucleotide polymorphysiums (SNPs) in CHI3L1 might be associated with some clinical allergy diseases. Genetic variations in CHI3L1 were identified and genotyped in 143 allergy adults and 36 non-allergy adults. The variations of CHI3L1 SNPs (-131C/G and -247C/T) were examined by three different PCR methods (1) DNA sequence analysis, (2) TaqMan analysis and (3) High Resolution Melt analysis. We recorded and compared these data by statistical analysis, and we investigated the relationship between adult allergy and CHI3L1 gene. Our preliminary studies have shown that, by comparing DNA sequence analysis with TaqMan analysis and HRM analysis, the sensitivity are 65.85 and 89.74% and the specificity are 82.35 and 94.34% respectively. The variation of -131C/G promoter region had P values of <0.001, strongly supporting that the -131C/G polymorphism in the CHI3L1 is associated with high allergy risk. These results agreed well with those of the European researchers, the variation of -247C/T promoter region had a P values of >0.05, indicating that the SNP in the area CHI3L1 -247 is not associated with atopy. However, our results were different from those obtained by the Korean researchers. In follow-up studies we plan to collect more clinical specimens and do more clinical experiments to characterize the relationship between CHI3L1 gene and allergy.