Baculovirus Expression of Human Parvovirus B19 Structural Protein
碩士 === 中山醫學大學 === 免疫學研究所 === 99 === Previous studies have indicated that the capsids of human parvovirus B19 are composed of VP1 and VP2 proteins, also expression of VP2 alone in eukaryotic expression system could self-assemble into empty capsids, no matter in size, appearance, antigenicity or immun...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2011
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/46574514128498032351 |
| Summary: | 碩士 === 中山醫學大學 === 免疫學研究所 === 99 === Previous studies have indicated that the capsids of human parvovirus B19 are composed of VP1 and VP2 proteins, also expression of VP2 alone in eukaryotic expression system could self-assemble into empty capsids, no matter in size, appearance, antigenicity or immunogenicity are similar to native virions. The empty capsids which without genetic materials are called virus-like particles (VLPs). We used Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus expressing B19 VP2 capsid proteins. Polymerase chain reaction, Western Blot and indirect immunofluorescence assay were used to identify the VP2 proteins in infected cells. We hope to employ the platform of VP2 proteins expression for B19 diagnosis and vaccine development in the future.
|
|---|